A drop-down menu will appear with two file type options for saving. To save it click “Save Matrix” on the matrix editor window. Therefore, resetting any edited values will revert the values back to the same as the original acquisition matrix.ĥ) Once finished you can save your new matrix file to use with another workspace or export to CSV for further analysis. when “resetting” the edited FlowJo matrix, remember that it was simply a duplicate of the initial acquisition matrix.To remove the altered compensation matrix from a sample or samples, simply go back to the matrix editor and drag the original (unedited) version of the compensation matrix back on to a sample or group of samples.
#APPLY MATRIX FLOWJO 10 UPDATE#
#APPLY MATRIX FLOWJO 10 SERIES#
We have entered a series of values (1 to 100) in the FITC channel to exemplify this (see image below, top row). The higher the value, the darker the shades of yellow/orange will be applied. The color of the box will change depending on the input value. If you are editing the acquisition- defined matrix, the default name will be “Acquisition-defined-copy” and will be assigned a new color (default is blue).Ĥ) Mouse over and click in a box in the matrix window to edit the existing values. Hit “Enter” or “Return” on the keyboard to finalize the value. Therefore, a copy must be created in FlowJo that can be edited.Ģ) Click “Edit” to make a new (live) matrix and modify any of the matrix spillover values.ģ) A drop- down menu will then ask you if you would like to continue editing the acquisition matrix, click “Yes”.Ī new matrix will be created and immediately applied to all samples in the workspace. Acquisition matrices are usually grey by default.ĭouble-clicking the grid opens the matrix editor :įlowJo does not allow direct editing of the acquisition matrix to maintain the integrity of the FCS standard since these compensation values are stored within the file. If this gird appears when the data are first loaded, this means there is a compensation matrix embedded in the file and FlowJo has applied it. The color grid indicates that a compensation matrix has been applied to the sample. To alter an acquisition matrix on a file, please follow these steps:ġ) Double click on the grid next to the sample. You can also create an entirely new matrix with parameters of your choosing (See “Creating a New Matrix File” below). You can duplicate an existing matrix, which first must be edited and then applied to samples in the workspace.Any changes to spillover values are immediately applied to your samples in the workspace. You can edit an existing matrix, which creates a copy of the existing acquisition matrix and a live compensation node in the workspace.The two differ as to how they are applied to samples in the workspace. There are two of different ways in FlowJo vX to alter spillover values in an existing acquisition matrix. Good compensation matrices are derived from good compensation controls! Visual manipulations will likely only introduce error in the compensation calculation. Please Note: Altering these spillover values is not recommended unless it is done empirically. They are read and applied by FlowJo to the files automatically when the files are loaded. Chapter 1 – Editing Acquisition-Defined Compensation Matrices CHAPTER 1 – Altering an acquisition matrixĪcquisition matrices are defined by the $SPILL or $COMP keyword embedded within the fcs files.
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